Biology Flash Cards

Term:

Test of Recessivity

Description:

By crossing a mutant with wild-type, a geneticist can determine if the phenotype (observed trait) of the mutant is recessive or dominant. If the trait is recessive, the offspring will not show the phenotype because they will be heterozygotes. A geneticist will often do the Test Of Recessivity, followed by a Test of Complementation, followed by a Test of Epistasis

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Term	Description	SeeAlso
XGAL	Substance used for investigating the Lac Operon. XGAL cannot induce the Lac Operon, but it is a substrate for Beta-Galactosidase, and turns blue when it is broken down by Beta-Galactosidase. This enabled biochemists to study the Lac Operon with a substance that didn't induce the Lac Operon, but was a substrate for the Lac Operon (Lactose is both an inducer and substrate of the Lac Operon). Because it turns blue when broken down, this substance helped biochemists find Constitutive Mutants that always produced Beta-Galactosidase even when Lactose was not present.	Lac Operon, IPTG, Constitutive Mutants

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