| Term: |
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| Description: |
A scientist wishing to sequence an entire genome can do so by performing variations on the following procedure: 1.) Create a library of clones via DNA Cloning; usually the scientist breaks up the library into medium-sized strands of 100,000 base pairs (perhaps cloning these sequences in BACs) 2.) Take one of the BACs from step 1, and break it up into even smaller segments which are suitable for gel electrophoresis (< 1000 base pairs). 3.) Sequence the short segments from Step 2 using Gel Electrophoresis. 4.) Use computer programs to find the overlaps in the short segments from Step 3, and thus put the medium-sized sequences in order. 5.) Put the medium-sized sequences from step 4 in order, perhaps by again using computer programs to find overlaps between the BAC sequences. This technique was developed by Sanger circa 1980.
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| See Also: |
Library, DNA Cloning, BAC
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