| Term: |
|
| Description: |
A technique used by biochemists to analyze a clone. A biochemist make take a gene sequence which she wishes to analyze, cuts it with a certain restriction enzyme, and then uses gel electophoresis to analyze the sizes of the cut pieces. She then uses a second restriction enzyme to cut the gene, and again uses gel electorphoresis. She then cuts the gene with both restriction enzymes, and again performs gel electrophoresis. Entering the observed results from the many gel electrophoresis procedures into a computer program, she can determine the locations of the restriction sites. This is called Restriction Mapping.
|
| See Also: |
Gel Electrophoresis, Cloning
|